Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii using liquid chromatography-tandem mass spectrometry

摘要

Multiple expressed β-lactamases could be identified simultaneously using LC-MS/MS. The β-lactamases most likely responsible for the resistant phenotype were detected in all isolates that were resistant to carbapenems and/or ceftazidime. The excellent correlation between the detection of the β-lactamase proteins - rather than PCR detection of the corresponding genes - with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.