Cytosep membranes have minimal impact on MS analysis of plasma obtained from whole blood using the membrane 1. Human fibrinogen on the membrane aids in the separation of red blood cells from whole blood. 2. The signal reduction caused by extraction using fibrinogen treated membranes needs to be addressed. A reproducible amount of plasma can be obtained from whole blood under a variety of extraction conditions using a gravity filtration membrane Calibration curves prepared using the extraction method are similar to those prepared using centrifuged plasma and an identical paper spray MS analysis. 1. Curve needs to be repeated with plasma calibrators rather than whole blood calibrators. 2. The accuracy of the method needs to be verified.
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