Improved Characterization of Highly Conserved Proteins using Middle-Down 193 nm Ultraviolet Photodissociation

摘要

93 nm UVPD provides unambiguous phosphosite localization for large peptides produced by tryptic digestion of TFIIH exposed drosophila RNA Pol II CTD. Lys-C digestion of IgG mixtures reduces sample complexity compared to conventional bottom-up methods and generates highly informative full-length CDR-H3 peptides 193 nm UVPD provides comprehensive characterization of diagnostic CDR-H3 peptides for improved identification of antigen-specific IgGs.