Ion Mobility Spectrometry-Hydrogen Deuterium Exchange Mass Spectrometry of Anions: Complementing Cation Studies

摘要

HDX data for protonated and deprotonated peptides and proteins are complementary in nature. Collision induced dissociation of protein complexes to their subunits in negative ion mode is potentially of greater utility than positive ion mode. For deprotonated insulin no interchain H/D scrambling observed.