Towards absolute quantification in glycomics facilitating new labeling strategies for Pichia pastoris glycans

机译：促进拟合毕赤酵母聚糖的新标签策略的含量绝对量化

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LC-ICPMS successful holmium integration and major product Ho-DOTA MALDI-MS shows derivatization of GlcNAc Transfer to bigger sugar chains and glycans Need to remove of Ho-DOTA label excess for quantification purposes and address dimer and trimer formation issue Ho-DOTA complexes offer the potential for sensitive LC-ICPMS based glycan assays and absolute quantification strategies

机译：LC-ICPMS成功的钬整合和主要产品Ho-Dota Maldi-MS显示GlcNAC转移到更大的糖链和聚糖的衍生化需要去除HO-DotA标签过量以进行量化，并且地址二聚体和三聚体形成问题HO-DOTA复合物提供基于敏感的LC-ICPMS的糖类测定和绝对量化策略的潜力

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2015年||共1页
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Evelyn Rampler; Gunda Koellensperger;
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1. A novel, simplified strategy of relative quantification N -glycan: Quantitative glycomics using electrospray ionization mass spectrometry through the stable isotopic labeling by transglycosylation reaction of mutant enzyme Endo-M-N175Q [J] . Qing Shi, Ryugo Hashimoto, Tadamune Otsubo, Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis . 2018,第期

机译：一种新颖的，简化的相对定量N-Glycan策略：使用电喷雾电离质谱通过突变酶Endo-M-N175Q的常糖基化反应通过稳定同位素标记的定量糖。
2. Chemo-Enzymatic Synthesis of C-13 Labeled Complex N-Glycans As Internal Standards for the Absolute Glycan Quantification by Mass Spectrometry [J] . Echeverria Begona, Etxebarria Juan, Ruiz Nerea, Analytical chemistry . 2015,第22期

机译：化学-酶法合成C-13标记的复杂N-聚糖作为内标的质谱定量法
3. Quantification by isobaric labeling (QUIBL) for the comparative glycomic study of O-linked glycans [J] . Botelho JC, Atwood JA, Cheng L, International journal of mass spectrometry . 2008,第2a3期

机译：通过等压标记（QUIBL）定量进行O联聚糖的比较血糖研究
4. Towards absolute quantification in glycomics facilitating new labeling strategies for Pichia pastoris glycans [C] . Evelyn Rampler, Gunda Koellensperger ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：促进拟合毕赤酵母聚糖的新标签策略的含量绝对量化
5. Relative Quantification of N-linked Glycans in Complex Mixtures via Stable Isotope Labeling and Enhanced Analysis by Liquid Chromatography Coupled Online to Mass Spectrometry [D] . Walker, Steven Hunter 2013

机译：通过稳定同位素标记和通过液相色谱-质谱联用技术增强分析的复杂混合物中N-连接聚糖的相对定量
6. Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT): A Novel Glycan Relative Quantification Strategy [O] . S. Hunter Walker, Amber D. Taylor, David C. Muddiman -1

机译：同位素糖化标签标记时的个性化归一化（INLIGHT）：一种新型的糖化相对定量策略
7. An improved comprehensive strategy for deep and quantitative N-glycomics based on optimization of sample preparation, isotope-based data quality control and quantification, new N-glycan libraries and new algorithms [O] . Yudong Guan, Jiaxiang Hu, Weiqian Cao, 2020

机译：基于样品制备的优化，基于同位素的数据质量控制和量化，新的N-Glycan库和新算法，改善了深度和定量N-Glycomics的综合策略

Towards absolute quantification in glycomics facilitating new labeling strategies for Pichia pastoris glycans

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