The non-targeted metabolomics of rice profiling analysis by Agilent 1290UHPLC/6550 iFunnel Q-TOF system

摘要

Our objective to evaluate an LC/MS method with the different extraction buffer for grain and leaf of rice. Agilent developed excellent separation condition for isomeric compound. (Figure 3) The good mass accuracy and relative standard deviations have be shown in Figure 2. Agilent MPP 13.0 has shown the power statistical calculation for PCA and Clustering analysis in Figure 4 and 5. This study can be data visualizations for up and down regulation of metabolites in grain and leaf with heat map. There are 220 metabolites were annotated/identified by Agilent's library.