Integration of amino acid, acylcarnitine and steroids analysis in single FIA/LC-MS/MS platform

摘要

This platform is an effective tool for an initial screening and able to minimize sample consumption down to 1 uL. However, the use of a high performance LC/MS/MS system is highly recommended in order to achieve the appropriate level of sensitivity for the steroids especially. Using high performance LC/MS/MS (LCMS-8050, Shimadzu Corporation, Japan), the observed limit of detection (LOD) for the analysis of 17-hydroxyprogesterone was 0.19 ng/mL.