New in vivo RNA-binding architectures discovered by RBDmap

摘要

The RNA interactomes of HeLa and HEK293 cells jointly comprise 1106 RNA-binding proteins (RBPs)[1,2], with almost half of these lacking well-defined RNA-binding domains (RBDs), suggesting the existence of numerous unknown RNA-binding architectures. Here, we report on "RBDmap", a new method built on interactome capture [3], to comprehensively identify the RBDs of native RBPs in HeLa cells.