Quantitation of Pain Management Drugs Using Ultra-Fast Liquid Chromatography/Mass Spectrometry in Human Plasma Utilizing Positive/Negative Polarity Switching

摘要

Pain management is a branch of medicine employing an interdisciplinary approach for easing suffering and improving the quality of life of those living with pain. We herein describe a novel five minute LC-MS/MS assay for quickly identifying and quantifying drugs (a large pain panel) in human plasma. Utilizing ultra fast positive and negative polarity switching with a triple quadrupole mass spectrometer, it is demonstrated that both positive and negative ions from many drug classes in human plasma can be easily quantified in a single injection, with chromatographic peak widths spanning only 3-4 seconds.