Application of ESI-LC-MS/MS to mouse pharmacokinetic studies using serial and parallel blood sampling techniques

摘要

We have established a technique for serial sampling from one mouse, which generated the same quality of data as traditional parallel sampling. The animals did not show any observed adverse effect, suggesting the amount of blood loss is tolerable to the animals. Although not significant, there are differences in some PK parameters generated from these two methods for some compounds. Further studies are needed to understand the factors that caused those differences. Each sampling method has its own advantages and disadvantages (3). If the number of animals and the amount of test compound are an issue, the serial sampling method provides an alternative way to conduct mouse PK at early drug discovery stage.