Analysis of the Functional Role of Lys-11 in Polyubiquitin Chain Formation Using Quantitative Mass Spectrometry

摘要

The topologies of poly-ubiquitin chains in the low and high ubiquitin conjugates were studied using proteomic techniques. Ubiquitin linkages using K48 and K63 are the primary ubiquitination sites of the low ubiquitin conjugates. In the high Ub conjugates, Ubiquitin linkages using K11 is the critical site of poly-ubiquitin chain formation. The biochemical and physiological roles of Ubiquitin linkages using K11 in ubiquitin chain formation are under investigation.