Comparative studies of Protein Glycosylation Using Isotope Labeling and Electrospray Linear Ion Trap MAss Spectrometry

摘要

Reductive methylation is easily completed without any special reagents or reaction equipment and no significant side reactions occur during the labeling. Isotopic reductive methylation was found to be a feasible method for measuring the changes of site-specific glycosylation. Since the detected target was glycopeptides, the information of glycosylation site and glycoprotein pattern related to each glycosylation sites can be preserved.