Measurement of human protein turnover in vivo from tracheal aspirates using targeted proteomics

摘要

We demonstrate sufficient experimental precision to calculate the fractional breakdown rate (FBR) of SP-B in tracheal aspirates of neonatal patients with RDS. Furthermore, the measurement of an infused tracer into human proteins in vivo using our approach is generalizable to other proteins. Data showed that patients have different protein half-life (T1/2) and the results are being correlated with patients phenotypes.