Biomarker Discovery Of Amniotic Fluid From Patients With Posterior Urethral Valve Syndrome Using Proteomics Strategies

摘要

Posterior Urethral Valve (PUV) syndrome is one of numerous embryonic pathologies, which can cause neonatal morbidity and mortality. Currently there is no diagnostic tools available to predict and detect this disorder at the early stages of pregnancy. The goal of this study was to identify a panel of indicative and predictive protein biomarkers in amniotic fluid (AF) from fetuses using quantitative proteomics workflows on a QqT of and a MALDI TOF/TOF~(TM) mass spectrometer. Preliminary 2D electrophoresis (2DE) analysis showed striking differences between 2D protein maps of normal and pathological samples. It also showed complexity of protein content in amniotic fluid, which was reported for normal AF by other authors. We performed a comparison of protein content of AF between three patients with PUV syndrome and a healthy patient using the quantitative iTRAQ~(~R) reagents 8Plex analysis. As a result, we detected a panel of up- and down - regulated putative biomarkers. About 26 proteins were significantly changed between normal and pathological samples (data are under validation), while samples within PUV group or normal group are much more uniform. This confirms conclusions from our previous 2DE data. We present here the first comparative proteomic study of the PUV syndrome. Multiple differences in protein content, which we observed between PUV and normal amniotic fluid suggest that proteins which differ most are involved in pathological pathways. These putative protein biomarkers show promise for further classification, prediction and indicative monitoring of PUV disease during pregnancy. Further verification studies are being planned.