Column-switching reversed phase-hydrophilic interaction liquid chromatography/tandem mass spectrometry method for determination of free estrogens and their conjugates in river water

摘要

Dansyl chloride derivatization improved the detection sensitivity of free estrogens. HILIC separation improved the detection sensitivity of estrogen conjugates. Column-switching RPLC-HILIC allowed separation of these two groups of compounds in one run. The developed method could detect sub- to low- parts per trillion levels of free estrogens and estrogen conjugates in river water.