Comparison of Infrared Multiphoton and Collisionally Activated Dissociation of Supercharged Peptides in Ion Traps

摘要

One higher peptide charge state, not seen under conventional ESI-MS analysis, was produced when spiking m-NBA into working solutions of various model, phosphorylated, and tryptic peptides. When IRMPD was employed to dissociate these higher supercharged states, the most informative array of sequence ions was produced when compared to dissociation of lower charge states by IRMPD or upon dissociation of any charge states by CAD. Also, the total number of diagnostic sequence ions increased upon IRMPD of the higher charge states. However, often lower charge states led to the greatest number of sequence ions upon CAD. IRMPD of supercharged ions is anticipated to be particularly beneficial for larger peptides or proteins to maximize sequence coverage.