Pulsed Electron Beam Water Radiolysis: A Novel Method for Sub-Microsecond Hydroxyl Radical Protein Footprinting

摘要

Heavy oxidation of the protein can be achieved on a sub-microsecond time scale using a 2 MeV Van de Graaff electron accelerator. Oxidation is completed on a microsecond timescale before large-scale protein unfolding can occur. Sites of oxidation were mapped on the solvent accessible surface of beta-lactoglobulin using LC-MS/MS.