Study of tazobactam inhibition of beta-lactamases by electrospray ionization mass spectrometry (ESI-MS) under near physiological conditions

摘要

The present study highlights the importance of adopting near physiological conditions in ESI-MS studies of protein-inhibitor complexes. In addition, our results show that the E-I complex formed from PC1 beta-lactamase and tazobactam dissociates further to form an inactive, dehydrated form of the enzyme. This particular inhibition mechanism has the distinct advantage that the enzyme is 'permanently' deactivated, without the need to deal with the regeneration (turnover) of the active enzyme.