Ligand Binding-Mass Spectrometry Methods for Understanding Macromolecular Drug Biotransformation and Impact on Immunoassay Quantification

摘要

LBMS technology The 3 examples show that LBMS is able to track the metabolic fate of Fc fusion proteins. A high-affinity selective anti-Fc MAb concentrates the relevant analytes/metabolites and removes matrix proteins, resulting in high signal-to-noise. Top down MS of metabolites provides structural information including truncation points with time. Reduction/alkylation prior to analysis increased resolution and mass accuracy of metabolite signals. Captured metabolites can be probed by either MALDI TOF or nanoESI MS. MALDI provides a faster, simpler readout while nanoESI MS provides better resolution.