Identification of candidate lung cancer biomarkers by proteomic analysis of representative cell cultures and human plasma

摘要

Development of molecular biomarkers for lung cancer is needed for the assessment of reduced harm products. It has been previously observed that protein secretions increase during cancer metastasis (1,2). A global proteomic investigation of in vitro cell cultures representing lung cancer disease progression (BEAS-2B (nontumorigenic), A549 (tumorigenic), and H292 (tumorigenic and locally invasive)) was conducted. Candidate biomarkers for lung cancer were identified using nano flow reverse phase capillary liquid chromatography (Ncap-RP-LC) coupled to a hybrid ion trap Fourier transform ion cyclotron resonance mass spectrometer (LTQ-FTMS). These candidate biomarkers were then compared to proteins from healthy individuals and lung cancer patients using strong cation exchange (SCX) LC and Ncap-RP-LC cupled to an LTQ-FTMS. Select proteins were observed in the in vitro secretome (specific to lung cancer cell lines) as well as in human plasma from lung cancer patients.