DEVELOPMENT OF A HIGH-THROUGHPUT COCKTAIL DRUG-DRUG INTERACTION SCREEN USING MALDI TANDEM MASS SPECTROMETRY

摘要

>1 order of improvement for throughput from ESI based screen. Sufficient sensitivity can be achieved for our DDI cocktail assay. Good reproducibility, averaged RSD <10percent. Comparable inhibition data between MALDI and ESI based screens. SPE plate can be re-used for at least 21 elutions to reduce the cost of sample preparation. Potentials of MALDI MS/MS not limited to DDI assay.