Analysis of post-translational modifications of histones using chromatography, ion mobility tandem mass spectrometry and customized bioinformatics tools

摘要

IMS-MS and IMS-MSMS was applied to histone analysis. Glu-C digested histone purification are amenable to 'middle down' analysis by IMS-MS and IMS-MS/MS. Modifications induced by TSA treatment could be mapped by IMS-MS/MS. IMS-MS/MS is helpful for charge state separation and improves data interpretation. In house developed bioinformatics tool assists interpretation of complicated IMS-MS/MS data.