A Glycomics Application for the Detection of Tissue-Specific Changes in N- and O-glycosylation in the Nematode Caenorhabditis elegans.

摘要

Caenorhabditis elegans is an important model for carbohydrate recognition, an essential step in processes including, development, aging, and infection[1]. Thus, the development of a semi-quantitative comparison method for multiple classes of glycoconjugates in a tissue-specific fashion is necessary[2]. In the present study we have developed a glycomics approach for the comparison of N- and O-glycans in biologically relevant cohorts of C. elegans glycosylation mutants. In wildtype strains the bacterium Microbacterium nematophilum causes infection of the cuticle in the anal region and surrounding tissues[3]. The C.elegans bus (bacterially unswollen) mutants are resistant to infection by this nematode pathogen. We have used these mutants to develop a method for comparison of whole nematode and tissue-specific glycosylation changes, extending previous methods developed for organism-wide glycosylation[4].