Validation of the Pourquier ELISA to detect antibodies against Mycobacterium avium subspecies paratuberculosis in individual milk samples and bulk milk samples of Dutch dairy cows and herds

摘要

The objective of the study was to determine the diagnostic performance of the Pourquier ELISA for detection of antibodies against Map in individual milk samples and in bulk milk samples as compared with serum samples/seroprevalence. Milk and serum samples were tested and results were interpreted according to the instructions of the manufacturer. For individual milk samples the relative sensitivity was estimated by testing a panel of paired serum-milk samples from seropositive cattle (n=80), and the specificity was estimated by testing a panel of 500 individual milk samples from Map-free certified herds. Feasibility of bulk milk testing was investigated by titrating 65 positive individual milk samples in negative milk. Also 400 bulkmilk samples from herds with a known within-herd seroprevalence were tested. Specificity of bulkmilk samples was investigated by testing 100 bulkmilk samples from Map-free certified herds.