Isolation of Guajacum bulnesia Volatile Oil by Supercritical Carbon Dioxide Extraction

摘要

Isolation of volatile concentrate from the wood of Guajacum bulnesia, has been obtained by supercritical extraction with carbon dioxide. To obtain a pure volatile extract devoid of cuticular waxes, the extraction products were fractionated in two separators operating in series, A good extraction process was obtained operating at 120 bar and 80 °C in the extraction vessel, at 120 bar and at -5 °C in the first separator and at a pressure between 20 and 15 bar and temperature in the range (10 - 20) °C in the second one. The composition of the volatile concentrate has been analyzed by GC/MS, the major component were a-guaiene, cis-P-guaiene and a-bulnesene. A comparison with the oils obtained by hydrodistillation is also given, GC -^sMS analysis on the extracts revealed differences between the volatile oil obtained from supercritical extraction and the one extracted by hydrodistillation. In the case of supercritical extraction the yield was of volatile oil was 0,7 % by weight of the charged material.