Separation of glucosinolates from Camelina seed meal via membrane and acidic aluminum oxide column

摘要

Heavy metal salt is usually used to precipitate protein during extraction of glucosinolates from Camelina seed meal. That precipitant is expensive and environmental harmful . Molecular weight cutoff 5000 Dalton ultrafiltration (UF) membrane was employed to remove protein after ethanol extraction of glucosinolates. Palladium chloride was used to react with glucosinolates and colored reactant was produced. Absorbance at 540 nm was measured to determine the glucosinolate content according to the absorbance of model glucosinolate Sinigrin. Preparative ion exchange chromatography, based on acidic aluminum oxide, was used to further purify glucosinolates. 0.2 mol/1, 0.5mol/l and 1.0 mol/1 NaCl buffer were used to recover the glucosinolates absorbed by acidic aluminum oxide. The results show that 1.0 mol/1 salt solution can get 97.1% glucosinolates recovered for permeate from UF. The glucosinolate content of the seed meal is 125.5 mg/g. High performance liquid chromatography was employed to analyze the efficiency of FPLC. It indicates that the major glucosinolates were appeared at retention time 22.2 and 24.6 min. Most impurities of UF permeate were removed by using FPLC.