DETERMINATION OF BASIC COMPOUNDS WITH PEROXYOXALATE CHEMILUMINESCENCE DETECTION

摘要

Chemiluminescence (CL) reactions require no excitation light source, and thus avoid interfere by light scattering. Since CL permits attainment of large signal-to-noise ratio, determination of wide range analytes can be assayed. Luminol and its analogues are most famous CL reagents, and directly produce emission of light due to oxidation reactions with oxygen species such as hydrogen peroxide (H2O2). CL reactions involving an energy transfer reaction is also well known. Oxalates react with H2O2 to yield intermediate peroxides, which produce light by energy transfer to a co-existing fluorophore.1 Therefore, combination of oxalate with a fluorophore is important to yield intense emission. A large number of fluorophores are detected by the CL reaction with aryloxalates and H2O2. Among aryloxalates, bis(2,4,6-trichlorophenyl)oxalate (TCPO) is most popular for the CL reaction. Fluorophores derived from target compounds and H2O2 in samples have been determined in this CL detection system. Since the CL reaction using TCPO and H2O2 proceeds in the presence of base catalyst, the determination of basic compounds may also be possible.