Retention of fungal enzyme activity in Penicillium, Cladosporium, Aspergillus and Acremonium samples

摘要

Fungal biomass can be determined by measuring the beta-N- acetylhexos-aminidase (NAHA) enzyme activity. NAHA, an enzyme present in fungal mycelium and spores, has been detected in active, dormant and non-viable cells. Very little information is available on the enzyme activity of different species and retention of the activity under various storage conditions. In this study, the enzyme activity of liquid and filter samples containing spores of four fungal species was monitored using fluorometric method. Results indicate that enzyme activity varies between species and some species are missing detectable enzyme activity even when viability is detected. The results showed that samples should be stored as dry filter samples; suspensions should be stored -22°C.