MONITORING AND CONTROL OF PHARMACEUTICAL PROTEIN PRODUCTION WITH SEQUENTIAL INTEGRATED DOWN STREAM PROCESSES

摘要

The methylotrophic yeast Pichia pastoris is used for expression of pharmaceutical proteins like l-8del MCP-1 and vMIP-n. Here the proteins are secreted and these extra cellular products open new strategies in production processes. The combining of complex cultivations and primary down stream steps with cross flow filtration units leads to integrated bioprocessing. The development of optimised sequential production strategies in research and industrial scale needs on-line measurement of substrates andproducts up to the recombinant protein itself. Full automated processes are designed to analyse integrated filtration steps for modeling of cross flow units and combined bioreaction. The transfer of research work into industrial pilot plant scale is also demonstrated.