New concepts for antioxidants treatments of sperm DNA structure alteration

摘要

The major factor affecting ICSI success and source of concern is sperm DNA quality i.e. essentially fragmentation and decondensation. Although this technique has shown a high efficiency, its full safety is far from demonstrated: the injection of spermatozoa with damaged DNA, in a system where natural selection mechanisms are bypassed, may lead to birth defect and transmission of genetic diseases or cancer (Aitken et al. 2003, Fernandez-Gonzales et al. 2008). ROS affect sperm DNA quality not only by inducing fragmentation but also through formation of DNA adducts. We recently found even in our best samples, beside 8oxo deoxyguanosine, 1, N6-ethenoadenosine and 1, N2-ethenoguanosine, arising from a reaction with 4-hydroxy-2-nonenal, the main aldehyde compound released during lipid peroxidation (Badouard et al. 2008).