Extended Northern Portuguese database on 21 autosomal STRs used in genetic identification

摘要

21 autosomal STRs are used in our laboratory in routine casework by using two commercially available STR kits (Applied Biosystems' Identifiler and Promega's Powerplex 16) and also an in-house multiplex system, used especially in deficient paternity cases, which amplifies four more loci (CD4, F13A01, FES and MBPB). Northern Portuguese population data are presented here extensively, including D2S1338 and D19S433 for the first time. Deviations from Hardy-Weinberg equilibrium were detected inD8S1179 and Penta D loci, but applying the Bonferroni correction for the number of loci analyse,the departure in both loci was not significant (0.05/21=0.0024). Both commercial STR kits share 13 loci but use different primer pairs, and so genotype inconsistencies may occur. For individuals genotyped as homozygotes with one kit and as heterozygotes with the other, the latter genotype was the one considered. The overall matching probability for the 21 STRs in our population sample is of 1 in 1.56 x 1024 individuals and combined power of exclusion of 0.9999999914.