Efficiency of different PCR-based marker systems in assessing genetic diversity among rye inbred lines

摘要

The aim of this study was to investigate the efficiency of ISSR, SSR and SAMPL marker systems in detecting genetic polymorphism among 30 rye inbred lines. Each marker system was able to discriminate among the materials analysed with the lowest value of average genetic similarity (GS) obtained with ISSR markers (0.2888) and the highest with SAMPLs (0.5381). EST-derived SSRs turned out to be less efficient in detecting genetic diversity then those from genomic libraries (average GS values 0.3814 and 0.3221, respectively). The average GS value for combined SSR data was 0.3569. The lack of correlations between similarity and cophenetic matrices obtained with different marker systems suggests that different systems should be used simultaneously for a genetic diversity study to exploit as many sources of polymorphism as possible.