Clonal propagation and improved phytoextraction activity of 8 poplar clones (Populus canescens) in vitro

摘要

In vitro phytoextraction ability of vegetative clones of Populus canescens including two transgenic clones {ggsll and lgl6) was studied in leaf disc cultures. Clone stability was determined by fAFLP (fluorescent labeled amplified fragment length polymorphism). In total, 679 AFLP fragments were identified. Only one of them was different (99.85 % genetic similarity). Presence of the gshl transgene in the transformed clones (ggsl 1 and Igl6) was detected in PCR reactions using gshl specific primers. Forthe study of phytoextraction activity leaf discs (8 mm) were exposed to a concentration series of ZnS0_4 (10~(-1) to 10~(-5) M) incubated on tissue culture media WPM for 21 days. Zn~(2+) showed phytotoxicity only at high concentrations (10~(-1) to 10~(-2) M). Transgenic poplars showed elevated heavy metal (Zn and Cu) uptake as compared to the non-transformed clones. These results suggest that transgenic poplars may be suitable for phytoremediation of soils contaminated with Zn~(2+).