Assessment for salinity tolerance through intergeneric hybridisation (Triticum durum x Aegilops speltoides)

摘要

The development of more salt tolerant crop plants is a reasonable goal for plant breeding programs. For that purpose, a moderately salinity-tolerant Triticum durum accession was crossed with a moderately salinity-tolerant Aegilops speltoides. 500 hybrid seeds were produced, 95 % of which germinated. The triploid hybrid embryos produced from them were cultured on agar No. 1. Chromosome doubling was induced by using 0.05 % colchicine. The resulting hexaploid plants grew to maturity and produced a considerable amount of seeds. The synthetic hexaploid was tested for salinity tolerance, grown at 0, 100, 125, 150, 160, 170, 180, and 200 mM NaCl in a standard nutrient solution for two weeks. The amphidiploid materials showed greater salinity tolerance thaneither parent, suggesting the presence of different genes for tolerance in the parents.