Characterization of a tymoviruses causing disease in diascia ornamental plants

摘要

Most of the diascia and nemesia ornamental plants purchased from nurseries around the Washington State and Oregon showed symptoms of vein yellowing and mottling on infected leaves. Infected plants also displayed reduced vigour. Diascia x hybrida 'Sunchimes coral' developed virus-like symptoms of mild mottling and yellowing. RNA was extracted from infected tissues following the Dunn & Hitchborn (1965) procedure, and the entire genome was cloned and sequenced.