The extraction, purification and identification of the antifungal substance produced by Streptomyces lydicus A02

摘要

According to the literature, 80% of the bioactive substances used as agricultural antibiotics are produced by species of Streptomyces from soil. So exploiting actinomycetes from soil is an important approach for developing agricultural biocontrol agents, especially fungicides. Streptomyces lydicus A02 was isolated from a forest soil in Beijing suburb. In a previous study, the strain presented a stable and strong antagonistic activity against many plant pathogenic fungi, such as Botrytis cinerea, Alternaria solani, Fusarium graminearum, Rhizoctonia cerealis, and others. In potted plant trials performed in the greenhouse, high control efficiencies towards vegetable grey molds (up to about 90%) were obtained with the sterile filtrate from fermented broth of strain A02. It is obvious that the strain is able to produce efficient antifungal substances. In this research, the active metabolic product was extracted, purified and identified.