NEW INSIGHTS INTO THE MACROMOLECULAR AND SUPRAMOLECULAR NATURE OF HUMIC SUBSTANCES:AN ULTRAFILTRATION-HPSEC STUDY

摘要

The majority of studies supporting the supramolecular nature of humic substances(HS)have been founded on HPSEC studies(Piccolo,2002).However,some authors question the real validity of this technique to study aggregation phenomena in molecular systems(Varga et al.,2000).In order to explore the possible supramolecular and/or macromolecular nature of HS,alkaline and acidified(acetic acid and HCI)-realkalinized samples of three HS with different origin have been studied using ultrafiltration and HPSEC as complementary techniques.