首页> 外文会议>Italian Conference on Supercritical Fluids and Their Applications >ON SILICA GELS IMMOBILIZED CELLULASE AS BIOCATALYST FOR HYDROLYSIS OF CARBOXYMETHYL CELLULOSE
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ON SILICA GELS IMMOBILIZED CELLULASE AS BIOCATALYST FOR HYDROLYSIS OF CARBOXYMETHYL CELLULOSE

机译:在硅胶上固定化纤维素酶作为生物催化剂,用于水解羧甲基纤维素

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Cellulase preparation Celluzyme 0.7T was immobilized on silica gels using standard sol-gel procedure. To compare the properties and activity of immobilized enzymes, gels were dried by two different drying routes: conventionally in the air at 40°C (xerogels) and supercritically with CO_2 at 40°C and 100 bar (aerogels). The properties of enzyme preparations in the form of aerogel and xerogel were compared at atmospheric pressure and at the same time with the properties of crude enzyme preparation. Study of cellulase preparation (Celluzyme 0.7T) activity in three different forms in SC CO_2 will be reported. The aerogel and xerogel cellulase preparations were successfully reused many times in the high-pressure batch system.
机译:使用标准溶胶 - 凝胶程序将纤维素酶制剂加固0.7T在硅胶上固定。为了比较固定化酶的性质和活性,用两种不同的干燥途径干燥凝胶:通常在40℃(Xerogels)的空气中,并在40℃和100巴(气凝胶)的CO_2上过度地使用CO_2。在大气压下比较了气凝胶和Xerogel形式的酶制剂的性质,同时与粗酶制剂的性能同时。将报道研究SC CO_2中三种不同形式的纤维素酶制剂(Celluzyme 0.7T)活性。在高压批量系统中成功地重复使用气凝胶和Xerogel纤维素酶制剂。

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