Mating Type Factor A and Mating Pheromone Production of Tremella fuciformis

摘要

Eight strains of Tremella fuciformis were studied. Intrastrain and interstrain cross between single spore isolated from the eight strains indicated that the mating type factor A were biallelic. The effects of culture condition on mating pheromone production by T. fuciformis spores was studied. Single factor and orthogonal tests indicated the optimal culture condition for mating pheromone production was sucrose 20 g/L, (NH_4)_2 SO_4 12 mmol/L, MgSO_4. · 7H_2O 0.25 g/L, KH_2PO_4 · 3H_2O 0.5 g/L,VB_1 0.2 mg/L,ZnSO_4 · 7H_2O 2 mg/L, CaCl_2.· 2H_2O 0.5 g/L, (NH_4)_6Mo_7O_(24) · 4H_2O 0.02 mg/L, 23°C ,pH 6.5, 140 r/min. In the optimal culture condition, the highest activity was detected at the culture time of 4 days. The mating pheromone was completely inactivated by the treatment with pepsin, suggesting that the mating pheromone produced by H32 is a kind of peptide.