首页> 外文会议>International Symposium on Off-Flavours in the Aquatic Environment >Volatile organic compound (VOC) analysis and sources of limonene, cyclohexanone and straight chain aldehydes in axenic cultures of Calothrix and Plectonema
【24h】

Volatile organic compound (VOC) analysis and sources of limonene, cyclohexanone and straight chain aldehydes in axenic cultures of Calothrix and Plectonema

机译:挥发性有机化合物(VOC)分析和柠檬烯,环己酮和直链醛的钙噻虫和Plectonema的轴烯培养物

获取原文

摘要

The volatile organic compounds (VOCs) in four standing cultures of axenic benthic cyanobacteria (Calothrix and Plectonema) were studied by GC/MS analysis. To verify the biogenic origin of the VOCs, ~(13)C-labelling experiments were performed, and the differences in labelling were used to differentiate between biogenic and non-biogenic sources. Strongly labelled biogenic compounds were derived from the shikimate (cresol, skatole), isoprenoid (β-cyclocitral, β-ionone, geosmin) and fatty acid pathways (5Z-heptadecene, 3-octanone, 1 -hexanol). New odour compounds derived from microorgansims were β-cyclogeraniol, dihydro-β-ionone and 2-hydroxy-2,6,6-trimethylcyclohexanone. The latter compound had already been found in lake water, but could not be assigned to a particular organism. Straight chain aldehydes (octanal, nonanal, decanal), cyclohexanone, cyclohexanol and limonene were unlabelled beyond their natural isotope ratio. Therefore, the biogenic origin of these compounds is doubtful despite their occurrence in axenic cultures. The observation that limonene was a racemate supports this view. The precursors and reactions that lead to the formation of these non-biogenic compounds are unknown and it may be that they are formed by the analytical procedures rather than then being present in the culture medium.
机译:通过GC / MS分析研究了四种常驻蓝色细胞(Calothrix和Plectonema)的四种常规培养物中的挥发性有机化合物(VOC)。为了验证VOC的生物起源,进行〜(13)C标记实验,并使用标记的差异来区分生物和非生物源。强烈标记的生物化合物衍生自Shikimate(甲酚,石棉),异戊二烯(β-环克罗氏菌,β-离子,土工蛋白)和脂肪酸途径(5Z-庚二烯,3-辛烷酮,1-己醇)。衍生自微型微生物的新气味化合物是β-环丙酮,二氢-β-离子和2-羟基-2,6,6-三甲基环己酮。后一种化合物已经在湖水中发现,但不能分配给特定的生物体。直链醛(辛烷值,壬醛,癸醛),环己酮,环己醇和柠檬烯未被揭开其天然同位素比。因此,尽管发生轴静脉培养物,但这些化合物的生物起源是可疑的。柠檬烯是外消旋物的观察结果支持这种观点。导致这些非生物化合物形成的前体和反应是未知的,并且可以通过分析方法而不是存在于培养基中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号