Molecular Profiling in Quiescent and VEGF-activated Human Tumor Derived Vascular Endothelial Cells (HTDEC)

摘要

Endothelial cells (EC) play a significant role in providing and shaping the tumor mi-croenvironment by production and release of regulatory cytokines. To investigate proteins involved in main biological path-ways of HTDEC we performed proteomic analysis of quiescent and VEGF-activat-ed HTDEC. Human umbilical vein endothelial cells (HU-VEC) served as a control. EC where incubated in serum- and growth fac-tor deprived medi-um. After 24hrs, VEGF was added for 6hrs, and ~(35)S-Me-thionine/Cysteine was provided for the last 2hrs. Determination of protein amount by fluorimag-ing of 2D-gels was combined with measurement of incorporated ~(35)S. We found significant differences in the protein synthesis profiles of both quiescent and VEGF activated HUVEC and HTDEC. These differences might result in new targets for antiangiogenic therapies.