Antimicrobial Resistance in Neisseria Gonorrhoeae Isolated from Patients in Jiangsu Province, China

摘要

[Objective] To realize antibiotic resistance amongst Neisseria gonorrhoeae isolated from patients in different cities of Jiangsu province, in order to instruct correct treatment in gonorrhea infection. [Methods] The in-vitro susceptibility test was performed in 95 strains of N. gonorrhoeae. The Minimum inhibitory concentrations (MICs) of penicillin, tetracycline, ciprofloxacin, levofloxacin, ceftriaxone, cefotaxime, cefepime and spectinomycin were determined by agar dilution test using GC agar base containing 1% defined supplement and serial dilutions of each agent. PPNG were identified by commercial strips for detection of β-lactamase. TRNG were identified by strains' MIC. TEM gene and TetM gene were detected by PCR assay. [Results] There were 57 isolates of N. gonorrhoeae from Nanjing, 53 from Wuxi and 15 from Xuzhou. In all, there were 101 (80.8%) from symptomatic men and 24 (19.2%) from symptomatic women. Therefore, ciprofloxacin resistance was 100%, tetracycline resistance was 78.9%, and penicillin resistance was 68.4%, while spectinomycin resistance was detected as 1.1%. No resistance was detected against the third generation cephalosporines, including ceftriaxone, cefotaxime and cefepime. Of the 95 isolates, 52 (54.7%) were PPNG and penicillin MIC_(50)was 64μg/ml, 27 (28.4%) were TRNG and tetracycline MIC_(50) was 32 μg/ml. PPNG had high level TEM gene (96.3%) and TRNG had high level TetM gene (93.3%). [Conclusion] Antimicrobial resistance of N. gonorrhoeae was more serious in Jiangsu province. Ciprofloxacin may become a useless drug to treat gonorrhea infection in this province.