Use of a Silane-Coated Silica Particle Solution to Enhance Semen Quality of Stallions

摘要

Use of a proprietary silanated silica particle density gradient improved spermatozoal motility and morphology of stallions. Post-centrifugation resuspension in a milk-based extender improved spermatozoal motility more than pre-load dilution media. Sperm morphologic defects were separated differentially along the discontinuous gradient, yielding a higher percentage of morphologically normal sperm in the pellet. Discontinuous density gradients have been used to select populations of higher quality sperm for assisted reproductive techniques in humans. Density gradients separate cells (sperm) with differing specific gravities into different layers after centrifugation. The most commonly used discontinuous density gradient contained silica particles coated with polyvinylpyrroli-done.a This product has been removed from commercial use. An alternative discontinuous gradient that is used in humans, employs silane-coated silica particles in an isotonic solution for separation of sperm. The objective of thisstudy was to determine the optimal formulation and processing procedures necessary to use a similar product offered by the same company (labeled as EquiPure)c with equine sperm, based on in vitro sperm characteristics.