Spatio-temporal dynamics of genomicorganization and function in the mammaliancell nucleus

摘要

Recent developments in microscopy and computer imaging have led to a new viewof the cell nucleus based on genomic function. It is now known that genomicfunctions and factors which mediate these functions including DNA replication andtranscription, RNA transcript tracks and splicing factors are compartmentalized inthe cell nucleus in discrete domains (Berezney et al., 1995; Nickerson et al., 1995;Berezney, 2002; van Driel et al., 2003). Moreover, development of humanchromosome-specific DNA "paints" have demonstrated that the functional genomeitself is arranged into discrete chromosome specific territories in the interphasenucleus (Chevret et al., 2000; Cremer and Cremer 2001; Cremer et al., 2001; Paradaet al., 2003).