BIOCOMPATJBLE BIOMATERIALS FOR THE HUMAN CHONDROCYTE DIFFERENTIATION ESTIMATED BY RT-PCR METHOD

摘要

Biocompalibility of the biomaterials for the differentiation of the human articular cbondrocytes were estimated by reverse transcription-polymerase chain reaction (RT-PCR). We used five biodegradable polymers for culturing with human articular chondrocytes. In addition to these five materials, we also estimated aquous type of fullerene, namely C60 dimalonic acid (C60DMA). Cultures were carried out using micromass culture method for 4 weeks. Collagen type II, aggrecan and connexin43 gene levels were estimated using RT-PCR methods. Among the biomaterials, Poly glycolic acid (PGA) showed ihe highest expression level of the collagen type II gene. On the contraty, C60DMA showed the lowest expression level among six kinds of test substances. In the case of the aggrecan gene, PGA also showed the highest levels, and C60DMA showed the lowest ones. However, the expression patterns of the connexin 43 gene were different from previous two genes. Using the multi regression analysis was carried out between differentiation and these three gene expression levels. There was a high correlation between cellular differentiation and three gene expression levels.