Marrow mesenchymal stem cells cultured on alumina ceramics (From basic science to clinical applications)

摘要

Menchymal stem cells (MSCs) were obtained by culturing fresh rat and human bone marrow cells. The MSCs were then cultured on alumina ceramic disks in the medium supplemented with vitamin C, glycerophosphate and dexamethasone. The high alkaline phosphatase activities (ALP) of the cells on culture dishes as well as ceramic disks were observed. Patterns of ALP stain and mineralization on the dishes were similar to those on alumina ceramic disks. These results showed that alumina ceramic surface well supports the osteogenic differentiation of rat and human marrow MSCs. The differentiation leads to the appearance of active osteoblasts together with matrix formation; i.e. in vitro bone formation on the ceramic surface. Based on the findings, we initiated clinical trials using thus formed in vitro bone on alumina ceramics. After separation of MSCs from patient's aspirated bone marrow cells, we cultured the MSCs on the surface of artificial ankle prosthesis made by alumina ceramics and used for the treatments of the same patients.