A new enzymatic synthesis of pyruvic acid and L-lactic acid from acetaldehyde and carbon dioxide has been developed. The reaction of pyruvic acid synthesis utilizes reverse reaction of pyruvate decarboxylase in sodium bicarbonate buffer. The reaction proceeded at high pH, and gave better yield in higher concentration of buffer. The maximum yield was obtained in 500 mM sodium bicarbonate buffer at pH11. We also developed a one-pot, two-step enzymatic synthesis of L-lactic acid from acetaldehyde and carbon dioxide. The reaction employing reversal of pyruvate decarboxylase and hydrogenation of pyruvate by L-lactic dehydrogenase. The maximum yield was obtained at pH 9.5 in 500 mM sodium bicarbonate buffer.
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