Research on Relationship Between Aloe Polysac-charides and Active Oxygenic Free Radicals

摘要

7 aloe polysaccharide extracts are researched by chemical luminescence methods to see whether they have any relationship with active oxygenic free radicals. More attention is paid to two active components, CAP11 and CAP12. Results show that : except one, most of these extracts stimulate phagocyte to produce more active oxygenic free radicals, viz. they induce the phenomenon of "respiration erupt". Magnitude of the stimulation is accordant with their immune-enhancing ability. For CAP11 and CAP12, the ability of stimulation increases with the concentration. Luminous peak of CAP12 is earlier than CAP11. No difference is observed between the active polysaccharides with and without protein. This indicates that protein is not an indispensable part of aloe polysaccharide during the stimulation process. Whether kept original or deprived of protein, the active polysaccharides are all positive in annihilation of active oxygenic free radicals. The scavenging ratios increase with the amounts of polysaccharides added. Difference between these polysaccharides is very litter. Among all the active oxygenic free radicals tested, these active polysaccharides show higher ability in annihilation of • OH which is produced by VitC-CuSO_4-H_2O-2-0-Phen. system. SC_(50) (half scavenge concentration) is approximately 1.5 times of the reference sulfonylurea. The active polysaccharides show poor ability in annihilation of O_2~- · which is produced by pyrogallol self-oxidation system. SC_(50) is about 10 times of the reference cysteine. As for H_2O_2, SC_(50) is some 9 times of the reference cysteine. Reasons for such phenomenon are discussed.