Evaluation of antibody response to swine influenza virus subtypes H1N1 and H3N2 in vaccinated pigs by different test procedures

摘要

Swine influenza virus (SIV) serology is used to determine the status of herds, to monitor maternal antibody decay and to document seroconversion to vaccination with killed vaccines. Serological tests commonly available from diagnostic laboratories include hemaglutination inhibition (HI) tests for H1N1 and H3N2 and ELISA tests (IDEXX Herd Check H3N2 and H1N1 ELISA, Westbrook, Maine). The IDEXX HerdCheck H1N1 ELISA has been commonly used for vaccination compliance monitoring with Schering's influenza vaccines, MaxiVac Excell and MaxiVac Excell 3. Recently, however, reports from the field indicated that seroconversion to two doses of MaxiVac Excell was becoming difficult to detect with the IDEXX H1N1 ELISA. The purpose of this study was to investigateif there has been any variation in the IDEXX H1N1 ELISA over the past year and a half.