Chloroform-phenol mixed solvent for efficient segment condensation reaction of sparingly soluble protected petpides performed using solution or solid-phase method

摘要

We recently demonstrated the utility of a combined solid-phase and solution approach for protein synthesis by synthesizing green fluorescent protein (238 AAs) and human leptin (146 AAs).This procedure is based on performing the segment condensation reaction in solution employing a maximum protection strategy with Boc chemistry.